(* means:) Playable in Musou Mode, Free Mode and Challenge Mode. Others playable in only Free Mode and Challenge Mode.
Character | How to unlock
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| - Clear Musou Mode with three Wei characters.
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| - Defeat Cao Pi within 5 minutes on Sun Jian side of He Fei.
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| - Succeed in Battle Objective 2 on the Lu Bu side of Fan Castle.
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| - Clear Musou Mode with 1 character from each Kingdom.
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| - Succeed in Battle Objective 1 on the Lu Bu side of The Battle of Hu Lao Gate.
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| - Clear Musou Mode with 1 Wu character.
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| - Succeed in 2 battle objectives on the Shu side of Fan Castle and finish battle with Guan Ping alive.
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| - Succeed in Battle Objective 3 on the Wu side of The Battle of Chi Bi.
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| - Succeed in 1 battle objective on the Wei side of Ding Jun Mountain.
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| - Succeed in Battle Objective 1 on the Wei side of the Battle of Shi Ting.
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| - Clear Musou Mode with 3 Shu characters.
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| - Clear Musou Mode with Liu Bei, Cao Cao, Diao Chan, and Sun Jian.
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| - Succeed in 2 battle objectives on the Wei side of He Fei.
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| - Succeed in 2 battle objectives on the Wei side of Han Zhong Attack Defense Battle.
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| - Succeed in 3 battle objectives at Cheng Du battle and finish battle with Pang Tong alive.
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| - Succeed in Battle Objective 1 on the Sun Jian side of the Battle of Xia Pi.
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| - Clear Musou Mode with 3 Wu characters.
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| - Succeed in Battle Objective 1 on the Lu Bu side of The Battle of Chi Bi.
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| - Defeat him in the Battle for Wu Territory.
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| - Succeed in 2 battle objectives on the Shu side of WuZhang Plains and finish battle with Wei Yan alive.
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| - Succeed in 1 battle objective on the Shu side of Ding Jun Mountain.
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| - Succeed in Battle Objective 3 on the Wu side of the Battle of Shi Ting.
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| - He Fei, Wu side, Personally kill Cao Ren and Xu Chu.
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| - Succeed in 2 battle objectives on the Wei/Wu side of Fan Castle.
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| - As Wei, complete all 3 targets and capture Wu Chao before completing the stage.
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| - Succeed in Battle Objective 1 on the Wei side of the Battle of Wu Zhang Plains.
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| - Beat stage with completing all three targets in the Yellow Turban Rebellion.
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| - Clear Musou Mode with 1 Wei character.
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| - Succeed in Battle Objective 1 on the Lu Bu side of the Xu Du Invasion.
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| - Succeed in 2 battle objectives on the Shu side of Han Zhong Attack Defense Battle.
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| - Succeed in 3 battle objectives on the Shu side of Yi Ling.
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| - Clear Musou Mode with 1 Shu character.
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