| Biocard | How to unlock
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| - Beat Mission 1 on Easy difficulty or Normal difficulty.
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| - Beat Mission 2 on Easy difficulty or Normal difficulty.
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| - Beat Mission 3 on Easy difficulty or Normal difficulty.
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| - Beat Mission 4 on Easy difficulty or Normal difficulty.
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| - Beat Mission 5 on Easy difficulty or Normal difficulty.
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| - Collect all 25 Titan cards in Mission 1 on Easy difficulty or Normal difficulty.
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| - Collect all 25 Titan cards in Mission 2 on Easy difficulty or Normal difficulty.
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| - Collect all 25 Titan cards in Mission 3 on Easy difficulty or Normal difficulty.
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| - Collect all 25 Titan cards in Mission 4 on Easy difficulty or Normal difficulty.
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- 10. Brother Blood (regular white attire)
| - Beat Mission 6 in under 25 minutes on Normal difficulty.
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- 11. Brother Blood (cybernetic red attire)
| - Collect all 25 Titan cards in Mission 6 on Easy difficulty or Normal difficulty.
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| - Collect all 150 Titan cards in the game on Easy difficulty or Normal difficulty.
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| - Beat Mission 4 with a success rate of 80% or higher on Hero difficulty.
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| - Beat Mission 1 in under 20 minutes on Hero difficulty.
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| - Beat Mission 5 in under 20 minutes on Titans difficulty.
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| - Beat Mission 2 in under 20 minutes on Normal difficulty.
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| - Collect all 25 Titan cards in Mission 5 on Easy difficulty or Normal difficulty.
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| - Beat Mission 4 in under 20 minutes on Normal difficulty.
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| - Collect all 150 Titan cards in the game on Hero difficulty.
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| - Beat Mission 5 with a success rate of 80% or higher on Hero difficulty.
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| - Beat Mission 6 with a success rate of 80% or higher on Titans difficulty.
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| - Beat Mission 1 with a success rate of 95% or higher on Normal difficulty.
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| - Collect all 150 Titan cards in the game on Titans difficulty.
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| - Beat Mission 1 using only Robin on Normal difficulty.
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| - Beat the game on Titans difficulty.
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