| Achievement | How to unlock
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| Appetite Sated | Kill all the Ubers in the Hungry Spider level.
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| Bad Neighbours | Win level "New Home" with the challenge active.
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| Crickets and Spiders and Ants | Win level "Subjugation" with the challenge active.
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| Enemy Of My Enemy | Get a cricket to kill a funnel web spider in level "Subjugation".
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| First Innings | Win the level "New Home" before ten mole crickets have attacked with the challenge active.
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| Freedom - Hard | Win level "Subjugation" on Hard difficulty.
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| Freedom - Insane | Win level "Subjugation" on Insane difficulty.
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| Freedom - Medium | Win level "Subjugation" on Medium difficulty.
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| Freedom | Win level "Subjugation".
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| Get Your Own Eggs | Lose no more than one egg to the slave makers in level "Subjugation" on Insane difficulty.
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| I Can Eat 50 Eggs | Win the level "Subjugation" whilst losing at least fifty eggs to the slave makers on Insane difficulty with the challenge active.
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| Just Isn't Cricket | Win the level "New Home" after fifty mole crickets have attacked on Insane difficulty with the challenge active.
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| Moved In - Hard | Win level "New Home" on Hard difficulty.
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| Moved In - Insane | Win level "New Home" on Insane difficulty.
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| Moved In - Medium | Win level "New Home" on Medium difficulty.
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| Moved In | Win level "New Home".
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| NOPE | Kill ten funnel web spiders in the first 6 minutes, then win the level "Subjugation" on Hard difficulty.
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| Picky Eater | Win the level "New Home" with the last ten tunneled out creatures being woodlice on Insane difficulty.
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| Prison Break | Win level "Subjugation" on insane difficulty with the challenge active.
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| Quantity Not Quality | Do not upgrade any soldiers and win the level "New Home" on Medium difficulty.
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| Rapid Growth | Win the level "Subjugation" within sixteen minutes on Medium difficulty.
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| Speedy Setup | Win the level "New Home" on hard difficulty within seventeen minutes.
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| Stressful House Move | Win level "New Home" on Insane difficulty with the challenge active.
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| The First Experiment - Hard | Survive the first experiments. Win "Formicarium Challenge 1" on Hard difficulty.
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| The First Experiment - Insane | Survive the first experiments. Win "Formicarium Challenge 1" on Insane difficulty.
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| The First Experiment - Medium | Survive the first experiments. Win "Formicarium Challenge 1" on Medium difficulty.
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| The First Experiment | Survive the first experiments. Win "Formicarium Challenge 1".
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| Vanilla | Win "Formicarium Challenge 1" without unlocking black ants.
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