Collect all 45 hidden bee icons and get on the hidden Ura Route when you defeat Shooty at the end of the First Loop. Select "Yes" to start the Hidden Second Loop. In this loop, after Taisabachi is destroyed, Hibachi will attack you.
Open Second Loop
Collect 35 or more hidden bee icons. To find them, fire the laser so that the front edge of the beam passes over the icon and not continue. Defeat Shooty on Stage 5 and answer Longhena's question with the first option . This will take you to the Open Second Loop, with Taisabachi as the final Boss.
Alternately, complete the first loop without dying.