| Biocard | How to unlock
|
|---|
| 01. Robin | Beat Mission 1 on Easy difficulty or Normal difficulty.
|
| 02. Cyborg | Beat Mission 2 on Easy difficulty or Normal difficulty.
|
| 03. Raven | Beat Mission 3 on Easy difficulty or Normal difficulty.
|
| 04. Starfire | Beat Mission 4 on Easy difficulty or Normal difficulty.
|
| 05. Beast Boy | Beat Mission 5 on Easy difficulty or Normal difficulty.
|
| 06. Hive Thug | Collect all 25 Titan cards in Mission 1 on Easy difficulty or Normal difficulty.
|
| 07. Gizmo | Collect all 25 Titan cards in Mission 2 on Easy difficulty or Normal difficulty.
|
| 08. Jinx | Collect all 25 Titan cards in Mission 3 on Easy difficulty or Normal difficulty.
|
| 09. Mammoth | Collect all 25 Titan cards in Mission 4 on Easy difficulty or Normal difficulty.
|
| 10. Brother Blood (regular white attire) | Beat Mission 6 in under 25 minutes on Normal difficulty.
|
| 11. Brother Blood (cybernetic red attire) | Collect all 25 Titan cards in Mission 6 on Easy difficulty or Normal difficulty.
|
| 12. Terra | Collect all 150 Titan cards in the game on Easy difficulty or Normal difficulty.
|
| 13. Bumblebee | Beat Mission 4 with a success rate of 80% or higher on Hero difficulty.
|
| 14. Speedy | Beat Mission 1 in under 20 minutes on Hero difficulty.
|
| 15. Más y Menos | Beat Mission 5 in under 20 minutes on Titans difficulty.
|
| 16. Mad Mod | Beat Mission 2 in under 20 minutes on Normal difficulty.
|
| 17. Control Freak | Collect all 25 Titan cards in Mission 5 on Easy difficulty or Normal difficulty.
|
| 18. Puppet King | Beat Mission 4 in under 20 minutes on Normal difficulty.
|
| 19. Mumbo Jumbo | Collect all 150 Titan cards in the game on Hero difficulty.
|
| 20. Johnny Rancid | Beat Mission 5 with a success rate of 80% or higher on Hero difficulty.
|
| 21. Cinderblock | Beat Mission 6 with a success rate of 80% or higher on Titans difficulty.
|
| 22. Slade | Beat Mission 1 with a success rate of 95% or higher on Normal difficulty.
|
| 23. Larry the Titan | Collect all 150 Titan cards in the game on Titans difficulty.
|
| 24. Red X | Beat Mission 1 using only Robin on Normal difficulty.
|
| 25. Trigon | Beat the game on Titans difficulty.
|