| Character | How to unlock
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|---|
| Evil Jackpot | Win the World Title with Jackpot (wearing the player two costume).
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| Ice Berg | Win World Title with Tiny. He has a solid record of 99 wins/KOs and 0 loss.
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| Jackpot (player two costume) | Win Professional Championship with him.
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| Joe Nitro | Win the Professional Championship with Mickey McFist.
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| Joe Nitro (alternate costume) | Win World Title with Joe Nitro..
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| Major Flag new costume | Win the Professional Championship with him, then highlight him and press Select. His shirt will be purple.
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| Miagaru new costume (yellow) | Win the World Title with him.
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| Miagaru player two costume (white) | Win the Professional Championship with him.
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| Psycho Tick | Win the Professional Championship using The Hammer.
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| Royal Pain new costume | Win the World Title with him. When done, highlight him and press Select. His skin will be orange.
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| Royal Pain player two costume | Win the Professional Championship with him, then highlight him and press Select. He will have pale skin.
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| Sgt. Drill | Win the Professional Championship using Major Flag.
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| Tiny new costume | Win the Professional Championship,with him, then highlight him and press Select. His skin will be gray.
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| Violent Violet | Win the Professional Championship with Knuckles Nadine.
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| Wendy Wacker | Win the World Title using Violent Violet.
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